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  • Description: serine protease, cleaves SpoIVFA, this results in pro-SigK processing/activation in the mother-cell, does also cleave CtpB

Gene name spoIVB
Essential no
Product serine protease
Function control of SigK activation
MW, pI 45 kDa, 8.842
Gene length, protein length 1275 bp, 425 aa
Immediate neighbours spo0A, recN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SpoIVB context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU24230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Self-cleaves 52-Val-|-Asn-53, 62-Ala-|-Phe-63 and 74-Val-|-Thr-75 at the N-terminus of SpoIVB (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Simon Cutting, London, UK homepage

Your additional remarks


Nathalie Campo, David Z Rudner
SpoIVB and CtpB are both forespore signals in the activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2007, 189(16);6021-7
[PubMed:17557826] [WorldCat.org] [DOI] (P p)

Thierry Doan, David Z Rudner
Perturbations to engulfment trigger a degradative response that prevents cell-cell signalling during sporulation in Bacillus subtilis.
Mol Microbiol: 2007, 64(2);500-11
[PubMed:17493131] [WorldCat.org] [DOI] (P p)

Nathalie Campo, David Z Rudner
A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.
Mol Cell: 2006, 23(1);25-35
[PubMed:16818230] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Tran C Dong, Simon M Cutting
The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis.
J Biol Chem: 2004, 279(42);43468-78
[PubMed:15292188] [WorldCat.org] [DOI] (P p)

Qi Pan, Richard Losick, David Z Rudner
A second PDZ-containing serine protease contributes to activation of the sporulation transcription factor sigmaK in Bacillus subtilis.
J Bacteriol: 2003, 185(20);6051-6
[PubMed:14526016] [WorldCat.org] [DOI] (P p)

Tran C Dong, Simon M Cutting
SpoIVB-mediated cleavage of SpoIVFA could provide the intercellular signal to activate processing of Pro-sigmaK in Bacillus subtilis.
Mol Microbiol: 2003, 49(5);1425-34
[PubMed:12940997] [WorldCat.org] [DOI] (P p)

Ngo T Hoa, James A Brannigan, Simon M Cutting
The Bacillus subtilis signaling protein SpoIVB defines a new family of serine peptidases.
J Bacteriol: 2002, 184(1);191-9
[PubMed:11741860] [WorldCat.org] [DOI] (P p)

N T Hoa, J A Brannigan, S M Cutting
The PDZ domain of the SpoIVB serine peptidase facilitates multiple functions.
J Bacteriol: 2001, 183(14);4364-73
[PubMed:11418578] [WorldCat.org] [DOI] (P p)

P Wakeley, N T Hoa, S Cutting
BofC negatively regulates SpoIVB-mediated signalling in the Bacillus subtilis sigmaK-checkpoint.
Mol Microbiol: 2000, 36(6);1415-24
[PubMed:10931291] [WorldCat.org] [DOI] (P p)

P R Wakeley, R Dorazi, N T Hoa, J R Bowyer, S M Cutting
Proteolysis of SpolVB is a critical determinant in signalling of Pro-sigmaK processing in Bacillus subtilis.
Mol Microbiol: 2000, 36(6);1336-48
[PubMed:10931284] [WorldCat.org] [DOI] (P p)

M Gomez, S M Cutting
Expression of the Bacillus subtilis spoIVB gene is under dual sigma F/sigma G control.
Microbiology (Reading): 1996, 142 ( Pt 12);3453-7
[PubMed:9004507] [WorldCat.org] [DOI] (P p)

I Bagyan, J Hobot, S Cutting
A compartmentalized regulator of developmental gene expression in Bacillus subtilis.
J Bacteriol: 1996, 178(15);4500-7
[PubMed:8755877] [WorldCat.org] [DOI] (P p)

S Cutting, A Driks, R Schmidt, B Kunkel, R Losick
Forespore-specific transcription of a gene in the signal transduction pathway that governs Pro-sigma K processing in Bacillus subtilis.
Genes Dev: 1991, 5(3);456-66
[PubMed:1900494] [WorldCat.org] [DOI] (P p)

B E Van Hoy, J A Hoch
Characterization of the spoIVB and recN loci of Bacillus subtilis.
J Bacteriol: 1990, 172(3);1306-11
[PubMed:2106508] [WorldCat.org] [DOI] (P p)