spo0E

168

Spo0A-P phosphatase, control of the phosphorelay

locus: BSU_13640

Molecular weight: 9.65 kDa

pI: 6.48

Protein length: 85 aa

Gene length: 258 bp

function: initiation of sporulation

product: Spo0A-P phosphatase

essential: no

synonyms: spo0E

List of homologs in different organisms, belongs to COG5804 (Galperin et al., 2021)

Gene

Coordinates: 1,430,684  1,430,941

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The protein

Protein family

• spo0E family (with [protein|search|ynzd ]and [protein|022933D89666CDD2ACE39BCBB78D349BAF8E899B|yisI], according to UniProt)

Structure

• [AF|P05043]

Paralogous protein(s)

• [protein|A2250786C40A23A5EBD830B3853DE5187B3D24EC|ynzD]

Expression and Regulation

Operons

• genes: [gene|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E]

  description: [Pubmed|2838724]

  regulation

  • induced under stress conditions ([protein|search|SigB]) [Pubmed|22210769]

  regulatory mechanism

  • [protein|E5110D9C36E8C55AFD1419987B14A53232165F20|abrB]: repression, [Pubmed|2504584], in [regulon|protein:E5110D9C36E8C55AFD1419987B14A53232165F20|abrB regulon]
  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok]: repression, of the [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]-dependent promoter [Pubmed|22524514], in [regulon|protein:1508770D5BCA5739D13E3ABD11CC5C103C07FF25|rok regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|1901567], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]
  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB]: sigma factor, [Pubmed|22210769], in [regulon|protein:580011DE5DC40EC9E7E0512791D328FAA010DCB8|sigB regulon]

  additional information

  • Spo0E is degraded by [protein|search|FtsH]

Biological materials

Mutant

• BKE13640 ([gene|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE13640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCACTTCACCCCTCGCC,  downstream forward: _UP4_TAGCGGCCTATCAGATGCAT
• BKK13640 ([gene|A574974B6F4FF46DC69E03AE021651C67089866F|spo0E]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK13640 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTCACTTCACCCCTCGCC,  downstream forward: _UP4_TAGCGGCCTATCAGATGCAT

labs

• [wiki|Tony Wilkinson], York University, U.K. [http://www.york.ac.uk/depts/chem/staff/ajw.html homepage]

References

Reviews

• 19995980,35638784

Original Publications

• 2838724,1901567,8127878,11112444,11679073,12067336,15916600,15057450,17001075,18045868,19050850,19332814,22524514,22210769,2504584,22142536