Spo0B

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  • Description: sporulation initiation phosphotransferase

Gene name spo0B
Synonyms spo0D
Essential no
Product sporulation initiation phosphotransferase
Function initiation of sporulation
MW, pI 22 kDa, 4.963
Gene length, protein length 576 bp, 192 aa
Immediate neighbours obg, rpmA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Spo0B context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU27930

Phenotypes of a mutant

Arrest of sporulation at stage 0 (initiation) PubMed Central

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • Structure: 1F51 (complex with Spo0F), 1IXM
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • spo0B12, point mutation 85C>T (=R29W), available in BGSC 1S54
    • spo0B136, amber mutation 103A>T (=K35X), available in BGSC 1S16
    • spo0B580ts, point mutation 193G>A (=E65K), available in BGSC 1S90
    • spo0B581ts, point mutation 461G>A (=G154D), available in BGSC 1S91
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Kottayil I Varughese, Igor Tsigelny, Haiyan Zhao
The crystal structure of beryllofluoride Spo0F in complex with the phosphotransferase Spo0B represents a phosphotransfer pretransition state.
J. Bacteriol.: 2006, 188(13);4970-7
[PubMed:16788205] [WorldCat.org] [DOI] (P p)

Masaya Fujita, Richard Losick
The master regulator for entry into sporulation in Bacillus subtilis becomes a cell-specific transcription factor after asymmetric division.
Genes Dev.: 2003, 17(9);1166-74
[PubMed:12730135] [WorldCat.org] [DOI] (P p)

Sophie J Stephenson, Marta Perego
Interaction surface of the Spo0A response regulator with the Spo0E phosphatase.
Mol. Microbiol.: 2002, 44(6);1455-67
[PubMed:12067336] [WorldCat.org] [DOI] (P p)

J Zapf, U Sen, Madhusudan, J A Hoch, K I Varughese
A transient interaction between two phosphorelay proteins trapped in a crystal lattice reveals the mechanism of molecular recognition and phosphotransfer in signal transduction.
Structure: 2000, 8(8);851-62
[PubMed:10997904] [WorldCat.org] [DOI] (P p)

Y L Tzeng, X Z Zhou, J A Hoch
Phosphorylation of the Spo0B response regulator phosphotransferase of the phosphorelay initiating development in Bacillus subtilis.
J. Biol. Chem.: 1998, 273(37);23849-55
[PubMed:9726997] [WorldCat.org] [DOI] (P p)

C E Grimshaw, S Huang, C G Hanstein, M A Strauch, D Burbulys, L Wang, J A Hoch, J M Whiteley
Synergistic kinetic interactions between components of the phosphorelay controlling sporulation in Bacillus subtilis.
Biochemistry: 1998, 37(5);1365-75
[PubMed:9477965] [WorldCat.org] [DOI] (P p)

Y L Tzeng, J A Hoch
Molecular recognition in signal transduction: the interaction surfaces of the Spo0F response regulator with its cognate phosphorelay proteins revealed by alanine scanning mutagenesis.
J. Mol. Biol.: 1997, 272(2);200-12
[PubMed:9299348] [WorldCat.org] [DOI] (P p)

X Z Zhou, Madhusudan, J M Whiteley, J A Hoch, K I Varughese
Purification and preliminary crystallographic studies on the sporulation response regulatory phosphotransferase protein, Spo0B, from Bacillus subtilis.
Proteins: 1997, 27(4);597-600
[PubMed:9141138] [WorldCat.org] [DOI] (P p)

D Burbulys, K A Trach, J A Hoch
Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay.
Cell: 1991, 64(3);545-52
[PubMed:1846779] [WorldCat.org] [DOI] (P p)

U Bai, M Lewandoski, E Dubnau, I Smith
Temporal regulation of the Bacillus subtilis early sporulation gene spo0F.
J. Bacteriol.: 1990, 172(9);5432-9
[PubMed:2118512] [WorldCat.org] [DOI] (P p)

P Zuber, R Losick
Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis.
J. Bacteriol.: 1987, 169(5);2223-30
[PubMed:2437099] [WorldCat.org] [DOI] (P p)

F A Ferrari, K Trach, J A Hoch
Sequence analysis of the spo0B locus reveals a polycistronic transcription unit.
J. Bacteriol.: 1985, 161(2);556-62
[PubMed:3918016] [WorldCat.org] [DOI] (P p)