Difference between revisions of "Spo0A"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 
* inactivation of ''[[spo0A]]'' restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
 
* inactivation of ''[[spo0A]]'' restores beta-lactam resistance in a ''[[sigM]]'' mutant {{PubMed|22211522}}
 +
* altered cell death pattern in colonies {{PubMed|23012477}}
  
 
=== Database entries ===
 
=== Database entries ===
Line 166: Line 167:
 
==Other original Publications==
 
==Other original Publications==
 
'''Additional publications:''' {{PubMed|22412392,21326214,21435029,21478340,21622736,21949067,21980382,22211522}}
 
'''Additional publications:''' {{PubMed|22412392,21326214,21435029,21478340,21622736,21949067,21980382,22211522}}
<pubmed>14679239, 1569009 20413551 20404177 19528067, 19581368 , 8730857,2106683,3157992, 11254139, 12067336, 10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 20689749  1537790,9658000, 8509330,14762002, 1846779, 2981817, 9477965,8600030, 18840696, 1391039, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 14712656 17157871 15060025 9685500 10852876 21552330  22303282 22745669 </pubmed>
+
<pubmed>14679239, 1569009 20413551 20404177 19528067, 19581368 , 8730857,2106683,3157992, 11254139, 12067336, 10869437, 16385044,9287005, 8127878,9287022, 8231806, 1905258,16452424,2118505,8207022, 12176382, 9733708, 11069677,1391039,12730135, 2437099,8288522,16166384,9495766,2118512,1556084, 18296515,7768874, 3145384, 19114652 20689749  1537790,9658000, 8509330,14762002, 1846779, 2981817, 9477965,8600030, 18840696, 1391039, 14976210, 19207565 11679073 12270811 11112444 18978066 20154131 14712656 17157871 15060025 9685500 10852876 21552330  22303282 22745669 23012477 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:15, 1 October 2012

  • Description: phosphorelay regulator, initiation of sporulation

Gene name spo0A
Synonyms spo0C, spo0G, spoIIL, sof-1
Essential no
Product phosphorelay response regulator
Function initiation of sporulation
Gene expression levels in SubtiExpress: spo0A
Interactions involving this protein in SubtInteract: Spo0A
Metabolic function and regulation of this protein in SubtiPathways:
Biofilm, Nucleotides (regulation), Ammonium/ glutamate,
Central C-metabolism,Sugar catabolism, Phosphorelay, Stress,
tRNA charging,Lipid synthesis, Protein secretion
MW, pI 29 kDa, 5.989
Gene length, protein length 801 bp, 267 aa
Immediate neighbours yqiG, spoIVB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Spo0A context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Spo0A expression.png



















Categories containing this gene/protein

transcription factors and their control, phosphorelay, biofilm formation, phosphoproteins

This gene is a member of the following regulons

SigH regulon, Spo0A regulon

The Spo0A regulon

The gene

Basic information

  • Locus tag: BSU24220

Phenotypes of a mutant

  • inactivation of spo0A restores beta-lactam resistance in a sigM mutant PubMed
  • altered cell death pattern in colonies PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: receives phosphorylation from Spo0B, dephosphorylation by Spo0E, direct phosphorylation by KinC upon potassium leakage PubMed
  • Cofactor(s):
  • Effectors of protein activity:
    • the phosphorylation state affects DNA binding activity
    • interaction with MecA inhibits the transcriptional activity of Spo0A∼P PubMed

Database entries

  • Structure: 1QMP (with phosphorylated aspartate, Geobacillus stearothermophilus), 1FC3 (trans-activation domain, Geobacillus stearothermophilus), 1LQ1 (complex with DNA)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed by casamino acids PubMed
    • repressed by SinR overexpression PubMed
    • repressed by soj PubMed
    • expression increases when drowth rate declines PubMed
  • Regulatory mechanism:
    • Spo0A: transcription repression PubMed
    • Spo0A synthesis is subject to a post-transcriptional control mechanism such that translation of mRNAs originating from the SigA-dependent promoter is impeded due to RNA secondary structure PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

The Spo0A regulon

Masaya Fujita, José Eduardo González-Pastor, Richard Losick
High- and low-threshold genes in the Spo0A regulon of Bacillus subtilis.
J. Bacteriol.: 2005, 187(4);1357-68
[PubMed:15687200] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol. Microbiol.: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Other original Publications

Additional publications: PubMed