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Gene name sigA
Synonyms rpoD, crsA
Essential yes PubMed
Product RNA polymerase major sigma factor SigA
Function transcription
MW, pI 42 kDa, 4.634
Gene length, protein length 1113 bp, 371 aa
Immediate neighbours cccA, dnaG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SigA context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU25200

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: sigma-70 factor family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Elecia B Johnston, Peter J Lewis, Renate Griffith
The interaction of Bacillus subtilis sigmaA with RNA polymerase.
Protein Sci.: 2009, 18(11);2287-97
[PubMed:19735077] [] [DOI] (I p)

Xiao Yang, Seeseei Molimau, Geoff P Doherty, Elecia B Johnston, Jon Marles-Wright, Rosalba Rothnagel, Ben Hankamer, Richard J Lewis, Peter J Lewis
The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA.
EMBO Rep.: 2009, 10(9);997-1002
[PubMed:19680289] [] [DOI] (I p)

Steve D Seredick, George B Spiegelman
The Bacillus subtilis response regulator Spo0A stimulates sigmaA-dependent transcription prior to the major energetic barrier.
J. Biol. Chem.: 2004, 279(17);17397-403
[PubMed:14976210] [] [DOI] (P p)

Amrita Kumar, James A Brannigan, Charles P Moran
Alpha-helix E of Spo0A is required for sigmaA- but not for sigmaH-dependent promoter activation in Bacillus subtilis.
J. Bacteriol.: 2004, 186(4);1078-83
[PubMed:14762002] [] [DOI] (P p)

Amrita Kumar, Cindy Buckner Starke, Mark DeZalia, Charles P Moran
Surfaces of Spo0A and RNA polymerase sigma factor A that interact at the spoIIG promoter in Bacillus subtilis.
J. Bacteriol.: 2004, 186(1);200-6
[PubMed:14679239] [] [DOI] (P p)

Claudia Rollenhagen, Haike Antelmann, Janine Kirstein, Olivier Delumeau, Michael Hecker, Michael D Yudkin
Binding of sigma(A) and sigma(B) to core RNA polymerase after environmental stress in Bacillus subtilis.
J. Bacteriol.: 2003, 185(1);35-40
[PubMed:12486038] [] [DOI] (P p)

H Jarmer, T S Larsen, A Krogh, H H Saxild, S Brunak, S Knudsen
Sigma A recognition sites in the Bacillus subtilis genome.
Microbiology (Reading, Engl.): 2001, 147(Pt 9);2417-24
[PubMed:11535782] [] [DOI] (P p)

J Qiu, J D Helmann
Adenines at -11, -9 and -8 play a key role in the binding of Bacillus subtilis Esigma(A) RNA polymerase to -10 region single-stranded DNA.
Nucleic Acids Res.: 1999, 27(23);4541-6
[PubMed:10556308] [] [DOI] (P p)

J Ju, T Mitchell, H Peters, W G Haldenwang
Sigma factor displacement from RNA polymerase during Bacillus subtilis sporulation.
J. Bacteriol.: 1999, 181(16);4969-77
[PubMed:10438769] [] (P p)

C M Buckner, G Schyns, C P Moran
A region in the Bacillus subtilis transcription factor Spo0A that is important for spoIIG promoter activation.
J. Bacteriol.: 1998, 180(14);3578-83
[PubMed:9658000] [] (P p)

G Schyns, C M Buckner, C P Moran
Activation of the Bacillus subtilis spoIIG promoter requires interaction of Spo0A and the sigma subunit of RNA polymerase.
J. Bacteriol.: 1997, 179(17);5605-8
[PubMed:9287022] [] [DOI] (P p)

X Huang, F J Lopez de Saro, J D Helmann
sigma factor mutations affecting the sequence-selective interaction of RNA polymerase with -10 region single-stranded DNA.
Nucleic Acids Res.: 1997, 25(13);2603-9
[PubMed:9185571] [] [DOI] (P p)

K Fredrick, J D Helmann
RNA polymerase sigma factor determines start-site selection but is not required for upstream promoter element activation on heteroduplex (bubble) templates.
Proc. Natl. Acad. Sci. U.S.A.: 1997, 94(10);4982-7
[PubMed:9144176] [] [DOI] (P p)

J D Helmann
Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA.
Nucleic Acids Res.: 1995, 23(13);2351-60
[PubMed:7630711] [] [DOI] (P p)

Y L Juang, J D Helmann
Pathway of promoter melting by Bacillus subtilis RNA polymerase at a stable RNA promoter: effects of temperature, delta protein, and sigma factor mutations.
Biochemistry: 1995, 34(26);8465-73
[PubMed:7599136] [] [DOI] (P p)

S E Aiyar, Y L Juang, J D Helmann, P L deHaseth
Mutations in sigma factor that affect the temperature dependence of transcription from a promoter, but not from a mismatch bubble in double-stranded DNA.
Biochemistry: 1994, 33(38);11501-6
[PubMed:7918363] [] [DOI] (P p)

J C Rong, J D Helmann
Genetic and physiological studies of Bacillus subtilis sigma A mutants defective in promoter melting.
J. Bacteriol.: 1994, 176(17);5218-24
[PubMed:8071196] [] [DOI] (P p)

Y L Juang, J D Helmann
A promoter melting region in the primary sigma factor of Bacillus subtilis. Identification of functionally important aromatic amino acids.
J. Mol. Biol.: 1994, 235(5);1470-88
[PubMed:8107087] [] [DOI] (P p)

H L Carter, L F Wang, R H Doi, C P Moran
rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis.
J. Bacteriol.: 1988, 170(4);1617-21
[PubMed:3127379] [] [DOI] (P p)

C W Price, R H Doi
Genetic mapping of rpoD implicates the major sigma factor of Bacillus subtilis RNA polymerase in sporulation initiation.
Mol. Gen. Genet.: 1985, 201(1);88-95
[PubMed:2997585] [] [DOI] (P p)

Additional publication: PubMed