Difference between revisions of "ScoC"

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=Biological materials =
=Biological materials =
* '''Mutant:'''  
* '''Mutant:''' Strain OM213 of Ogura MA, et al (Pubmed [[http://http://www.ncbi.nlm.nih.gov/pubmed?term=15126467 15126467]]), genotype ''trpC2 scoC::pMutin-scoC leuC7'', available as [http://bgsc.org BGSC] 1A918
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab

Revision as of 18:02, 9 September 2012

  • Description: transcriptional repressor of genes expressed in the transition phase

Gene name scoC
Synonyms hpr, catA
Essential no
Product transcriptional repressor (MarR family)
Function transition state regulator
Gene expression levels in SubtiExpress: scoC
Interactions involving this protein in SubtInteract: ScoC
Regulation of this protein in SubtiPathways:
Biofilm, Alternative nitrogen sources
MW, pI 23 kDa, 5.188
Gene length, protein length 609 bp, 203 aa
Immediate neighbours yhaI, yhaH
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Hpr context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
ScoC expression.png

Categories containing this gene/protein

transcription factors and their control, transition state regulators, phosphoproteins

This gene is a member of the following regulons

AbrB regulon, SalA regulon, SenS regulon

The ScoC regulon

The gene

Basic information

  • Locus tag: BSU09990

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-3 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
    • expressed upon transition into the stationary phase (AbrB) PubMed
  • Additional information:

Biological materials

  • Mutant: Strain OM213 of Ogura MA, et al (Pubmed [15126467]), genotype trpC2 scoC::pMutin-scoC leuC7, available as BGSC 1A918
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Bindiya Kaushal, Salbi Paul, F Marion Hulett
Direct regulation of Bacillus subtilis phoPR transcription by transition state regulator ScoC.
J. Bacteriol.: 2010, 192(12);3103-13
[PubMed:20382764] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
A dual mode of regulation of flgM by ScoC in Bacillus subtilis.
Can. J. Microbiol.: 2009, 55(8);983-9
[PubMed:19898538] [WorldCat.org] [DOI] (I p)

Takashi Inaoka, Guojun Wang, Kozo Ochi
ScoC regulates bacilysin production at the transcription level in Bacillus subtilis.
J. Bacteriol.: 2009, 191(23);7367-71
[PubMed:19801406] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC.
Microbiology (Reading, Engl.): 2009, 155(Pt 1);142-149
[PubMed:19118355] [WorldCat.org] [DOI] (P p)

Prashant Kodgire, Madhulika Dixit, K Krishnamurthy Rao
ScoC and SinR negatively regulate epr by corepression in Bacillus subtilis.
J. Bacteriol.: 2006, 188(17);6425-8
[PubMed:16923912] [WorldCat.org] [DOI] (P p)

Eiji Kawachi, Sadanobu Abe, Teruo Tanaka
Inhibition of Bacillus subtilis scoC expression by multicopy senS.
J. Bacteriol.: 2005, 187(24);8526-30
[PubMed:16321961] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Atsushi Matsuzawa, Hirofumi Yoshikawa, Teruo Tanaka
Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE.
J. Bacteriol.: 2004, 186(10);3056-64
[PubMed:15126467] [WorldCat.org] [DOI] (P p)

Alejandro Sánchez, Jorge Olmos
Bacillus subtilis transcriptional regulators interaction.
Biotechnol. Lett.: 2004, 26(5);403-7
[PubMed:15104138] [WorldCat.org] [DOI] (P p)

Sasha H Shafikhani, Esperanza Núñez, Terrance Leighton
ScoC mediates catabolite repression of sporulation in Bacillus subtilis.
Curr. Microbiol.: 2003, 47(4);327-36
[PubMed:14629015] [WorldCat.org] [DOI] (P p)

A Koide, M Perego, J A Hoch
ScoC regulates peptide transport and sporulation initiation in Bacillus subtilis.
J. Bacteriol.: 1999, 181(13);4114-7
[PubMed:10383984] [WorldCat.org] [DOI] (P p)

P T Kallio, J E Fagelson, J A Hoch, M A Strauch
The transition state regulator Hpr of Bacillus subtilis is a DNA-binding protein.
J. Biol. Chem.: 1991, 266(20);13411-7
[PubMed:1906467] [WorldCat.org] (P p)

B C Dowds, J A Hoch
Regulation of the oxidative stress response by the hpr gene in Bacillus subtilis.
J. Gen. Microbiol.: 1991, 137(5);1121-5
[PubMed:1907636] [WorldCat.org] [DOI] (P p)

M A Strauch, G B Spiegelman, M Perego, W C Johnson, D Burbulys, J A Hoch
The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein.
EMBO J.: 1989, 8(5);1615-21
[PubMed:2504584] [WorldCat.org] (P p)

M Perego, J A Hoch
Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis.
J. Bacteriol.: 1988, 170(6);2560-7
[PubMed:3131303] [WorldCat.org] [DOI] (P p)