From SubtiWiki
Revision as of 15:54, 11 November 2013 by (talk)
Jump to: navigation, search
  • Description: inhibition of the pro-sigma-K processing machinery

Gene name bofA
Essential no
Product inhibitor of pro-SigK processing
Function control of SigK activation
Gene expression levels in SubtiExpress: bofA
Interactions involving this protein in SubtInteract: BofA
MW, pI 8 kDa, 9.32
Gene length, protein length 261 bp, 87 aa
Immediate neighbours yaaL, rrnA-16S
Sequences Protein DNA DNA_with_flanks
Genetic context
BofA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BofA expression.png

Categories containing this gene/protein

sigma factors and their control, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

  • Locus tag: BSU00230

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:
    • forespore outer membrane (according to Swiss-Prot)
    • integral membrane protein PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: expressed during sporulation PubMed
  • Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Simon Cutting, London, UK homepage

Your additional remarks


Nathalie Campo, David Z Rudner
A branched pathway governing the activation of a developmental transcription factor by regulated intramembrane proteolysis.
Mol Cell: 2006, 23(1);25-35
[PubMed:16818230] [] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [] [DOI] (P p)

Tran C Dong, Simon M Cutting
The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis.
J Biol Chem: 2004, 279(42);43468-78
[PubMed:15292188] [] [DOI] (P p)

Ruanbao Zhou, Lee Kroos
BofA protein inhibits intramembrane proteolysis of pro-sigmaK in an intercompartmental signaling pathway during Bacillus subtilis sporulation.
Proc Natl Acad Sci U S A: 2004, 101(17);6385-90
[PubMed:15087499] [] [DOI] (P p)

Tran C Dong, Simon M Cutting
SpoIVB-mediated cleavage of SpoIVFA could provide the intercellular signal to activate processing of Pro-sigmaK in Bacillus subtilis.
Mol Microbiol: 2003, 49(5);1425-34
[PubMed:12940997] [] [DOI] (P p)

G Hambraeus, C von Wachenfeldt, L Hederstedt
Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs.
Mol Genet Genomics: 2003, 269(5);706-14
[PubMed:12884008] [] [DOI] (P p)

David Z Rudner, Richard Losick
A sporulation membrane protein tethers the pro-sigmaK processing enzyme to its inhibitor and dictates its subcellular localization.
Genes Dev: 2002, 16(8);1007-18
[PubMed:11959848] [] [DOI] (P p)

O Resnekov, R Losick
Negative regulation of the proteolytic activation of a developmental transcription factor in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1998, 95(6);3162-7
[PubMed:9501233] [] [DOI] (P p)

E Ricca, S Cutting, R Losick
Characterization of bofA, a gene involved in intercompartmental regulation of pro-sigma K processing during sporulation in Bacillus subtilis.
J Bacteriol: 1992, 174(10);3177-84
[PubMed:1577688] [] [DOI] (P p)

K Ireton, A D Grossman
Interactions among mutations that cause altered timing of gene expression during sporulation in Bacillus subtilis.
J Bacteriol: 1992, 174(10);3185-95
[PubMed:1315731] [] [DOI] (P p)

S Cutting, V Oke, A Driks, R Losick, S Lu, L Kroos
A forespore checkpoint for mother cell gene expression during development in B. subtilis.
Cell: 1990, 62(2);239-50
[PubMed:2115401] [] [DOI] (P p)