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  • Description: flagella-associated protein, signal recognition particle-GTPase

Gene name flhF
Essential no
Product signal recognition particle-GTPase
Function placement and assembly of polar flagella
MW, pI 40 kDa, 8.719
Gene length, protein length 1098 bp, 366 aa
Immediate neighbours flhA, swrC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FlhF context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU16400

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: GTP-binding SRP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure: 2PX3 (complexed with GTP-Mg2+)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Gert Bange, Georg Petzold, Klemens Wild, Richard O Parlitz, Irmgard Sinning
The crystal structure of the third signal-recognition particle GTPase FlhF reveals a homodimer with bound GTP.
Proc. Natl. Acad. Sci. U.S.A.: 2007, 104(34);13621-5
[PubMed:17699634] [] [DOI] (P p)

Geeske Zanen, Haike Antelmann, Helga Westers, Michael Hecker, Jan Maarten van Dijl, Wim J Quax
FlhF, the third signal recognition particle-GTPase of Bacillus subtilis, is dispensable for protein secretion.
J. Bacteriol.: 2004, 186(17);5956-60
[PubMed:15317803] [] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol. Microbiol.: 2003, 50(5);1683-701
[PubMed:14651647] [] [DOI] (P p)

P B Carpenter, D W Hanlon, G W Ordal
flhF, a Bacillus subtilis flagellar gene that encodes a putative GTP-binding protein.
Mol. Microbiol.: 1992, 6(18);2705-13
[PubMed:1447978] [] [DOI] (P p)