Difference between revisions of "Sandbox"

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* '''Description:''' flagella-associated protein, signal recognition particle-GTPase <br/><br/>
+
* '''Description:''' lipoprotein, putative ABC transporter (solute binding protein) <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''flhF''
+
|''lplA''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
Line 10: Line 10:
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || signal recognition particle-GTPase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || placement and assembly of polar flagella
+
|style="background:#ABCDEF;" align="center"|'''Function''' || unknown
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 8.719  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 56 kDa, 5.607  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1098 bp, 366 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1506 bp, 502 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhA]]'', ''[[swrC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yetA]]'', ''[[lplB]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13513&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12529&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:flhF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:lplA_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU16400
+
* '''Locus tag:''' BSU07100
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10544]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10434]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' GTP-binding SRP family (according to Swiss-Prot)
+
* '''Protein family:'''
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[YtcQ]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
Line 76: Line 76:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PX3 2PX3] (complexed with GTP-Mg2+)
+
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q01960 Q01960]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P37966 P37966]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU16400]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU07100]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 93: Line 93:
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** repressed under conditions that trigger sporulation ([[Spo0A]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
** [[Spo0A]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/14651647 PubMed]
 
  
 
* '''Additional information:'''  
 
* '''Additional information:'''  
Line 120: Line 118:
 
=References=
 
=References=
  
<pubmed>1447978,17699634,,15317803,14651647, </pubmed>
+
<pubmed>7921237,10092453, </pubmed>

Revision as of 16:48, 2 July 2009

  • Description: lipoprotein, putative ABC transporter (solute binding protein)

Gene name lplA
Synonyms
Essential no
Product unknown
Function unknown
MW, pI 56 kDa, 5.607
Gene length, protein length 1506 bp, 502 aa
Immediate neighbours yetA, lplB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LplA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU07100

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YtcQ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J. Mol. Biol.: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

A Gómez, D Ramón, P Sanz
The Bacillus subtilis lipoprotein LplA causes cell lysis when expressed in Escherichia coli.
Microbiology (Reading, Engl.): 1994, 140 ( Pt 8);1839-45
[PubMed:7921237] [WorldCat.org] [DOI] (P p)