From SubtiWiki
Revision as of 13:34, 5 December 2012 by Awiechert (talk | contribs)
Jump to: navigation, search
Gene name rocR
Essential no
Product transcriptional activator
Function transcriptional activator of arginine utilization operons
Gene expression levels in SubtiExpress: rocR
Interactions involving this protein in SubtInteract: RocR
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 52.6 kDa, 6.08
Gene length, protein length 1383 bp, 461 amino acids
Immediate neighbours rocD, yyzO
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RocR expression.png

Categories containing this gene/protein

transcription factors and their control

This gene is a member of the following regulons

The RocR regulon

The gene

Basic information

  • Locus tag: BSU40350

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: transcription activation at SigL-dependent promoters of rocABC, rocDEF, and rocG
  • Protein family: Sigma-54 interacting transcription activator
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Sigma-54 factor interaction domain (143–372)
    • 2x nucleotid-binding domain (171–178),(233–242)
    • HTH motif (434–453)
  • Modification:
  • Cofactor(s): ornithine or citrulline are required for RocR-dependent transcription activation PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon: rocR (according to DBTBS)
  • Regulatory mechanism: autorepression
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

Your additional remarks


Kenji Manabe, Yasushi Kageyama, Takuya Morimoto, Tadahiro Ozawa, Kazuhisa Sawada, Keiji Endo, Masatoshi Tohata, Katsutoshi Ara, Katsuya Ozaki, Naotake Ogasawara
Combined effect of improved cell yield and increased specific productivity enhances recombinant enzyme production in genome-reduced Bacillus subtilis strain MGB874.
Appl Environ Microbiol: 2011, 77(23);8370-81
[PubMed:21965396] [] [DOI] (I p)

Naima Ould Ali, Josette Jeusset, Eric Larquet, Eric Le Cam, Boris Belitsky, Abraham L Sonenshein, Tarek Msadek, Michel Débarbouillé
Specificity of the interaction of RocR with the rocG-rocA intergenic region in Bacillus subtilis.
Microbiology (Reading): 2003, 149(Pt 3);739-750
[PubMed:12634342] [] [DOI] (P p)

R Gardan, G Rapoport, M Débarbouillé
Role of the transcriptional activator RocR in the arginine-degradation pathway of Bacillus subtilis.
Mol Microbiol: 1997, 24(4);825-37
[PubMed:9194709] [] [DOI] (P p)

S Calogero, R Gardan, P Glaser, J Schweizer, G Rapoport, M Debarbouille
RocR, a novel regulatory protein controlling arginine utilization in Bacillus subtilis, belongs to the NtrC/NifA family of transcriptional activators.
J Bacteriol: 1994, 176(5);1234-41
[PubMed:8113162] [] [DOI] (P p)