RecU

From SubtiWiki
Revision as of 07:11, 17 June 2011 by JGerwig (talk | contribs) (Biological materials)
Jump to: navigation, search
  • Description: DNA repair, homologous recombination and chromosome segregation; recognizes, distorts, and cleaves four-stranded recombination intermediates and modulates RecA activities

Gene name recU
Synonyms recG, prfA, yppB, jopB
Essential no
Product Holliday junction resolvase
Function DNA repair, homologous recombination and chromosome segregation
MW, pI 23 kDa, 9.511
Gene length, protein length 618 bp, 206 aa
Immediate neighbours yppC, ponA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RecU context.gif
This image was kindly provided by SubtiList




Categories containing this gene/protein

DNA repair/ recombination, cell envelope stress proteins (controlled by SigM, W, X, Y)

This gene is a member of the following regulons

SigM regulon

The gene

Basic information

  • Locus tag: BSU22310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP891 (recU::cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Cristina Cañas, Begoña Carrasco, Esther García-Tirado, John B Rafferty, Juan C Alonso, Silvia Ayora
The stalk region of the RecU resolvase is essential for Holliday junction recognition and distortion.
J. Mol. Biol.: 2011, 410(1);39-49
[PubMed:21600217] [WorldCat.org] [DOI] (I p)

Dawit Kidane, Begoña Carrasco, Candela Manfredi, Katharina Rothmaier, Silvia Ayora, Serkalem Tadesse, Juan C Alonso, Peter L Graumann
Evidence for different pathways during horizontal gene transfer in competent Bacillus subtilis cells.
PLoS Genet.: 2009, 5(9);e1000630
[PubMed:19730681] [WorldCat.org] [DOI] (I p)

Begoña Carrasco, Cristina Cañas, Gary J Sharples, Juan C Alonso, Silvia Ayora
The N-terminal region of the RecU holliday junction resolvase is essential for homologous recombination.
J. Mol. Biol.: 2009, 390(1);1-9
[PubMed:19422832] [WorldCat.org] [DOI] (I p)

Cristina Cañas, Begoña Carrasco, Silvia Ayora, Juan C Alonso
The RecU Holliday junction resolvase acts at early stages of homologous recombination.
Nucleic Acids Res.: 2008, 36(16);5242-9
[PubMed:18684995] [WorldCat.org] [DOI] (I p)

Warawan Eiamphungporn, John D Helmann
The Bacillus subtilis sigma(M) regulon and its contribution to cell envelope stress responses.
Mol. Microbiol.: 2008, 67(4);830-48
[PubMed:18179421] [WorldCat.org] [DOI] (P p)

Natalie McGregor, Sylvia Ayora, Svetlana Sedelnikova, Begona Carrasco, Juan C Alonso, Paul Thaw, John Rafferty
The structure of Bacillus subtilis RecU Holliday junction resolvase and its role in substrate selection and sequence-specific cleavage.
Structure: 2005, 13(9);1341-51
[PubMed:16154091] [WorldCat.org] [DOI] (P p)

Begoña Carrasco, Silvia Ayora, Rudi Lurz, Juan C Alonso
Bacillus subtilis RecU Holliday-junction resolvase modulates RecA activities.
Nucleic Acids Res.: 2005, 33(12);3942-52
[PubMed:16024744] [WorldCat.org] [DOI] (I e)

Humberto Sanchez, Dawit Kidane, Patricia Reed, Fiona A Curtis, M Castillo Cozar, Peter L Graumann, Gary J Sharples, Juan C Alonso
The RuvAB branch migration translocase and RecU Holliday junction resolvase are required for double-stranded DNA break repair in Bacillus subtilis.
Genetics: 2005, 171(3);873-83
[PubMed:16020779] [WorldCat.org] [DOI] (P p)

Begoña Carrasco, M Castillo Cozar, Rudi Lurz, Juan C Alonso, Silvia Ayora
Genetic recombination in Bacillus subtilis 168: contribution of Holliday junction processing functions in chromosome segregation.
J. Bacteriol.: 2004, 186(17);5557-66
[PubMed:15317759] [WorldCat.org] [DOI] (P p)

B Carrasco, S Fernández, K Asai, N Ogasawara, J C Alonso
Effect of the recU suppressors sms and subA on DNA repair and homologous recombination in Bacillus subtilis.
Mol. Genet. Genomics: 2002, 266(5);899-906
[PubMed:11810266] [WorldCat.org] [DOI] (P p)

S Fernández, A Sorokin, J C Alonso
Genetic recombination in Bacillus subtilis 168: effects of recU and recS mutations on DNA repair and homologous recombination.
J. Bacteriol.: 1998, 180(13);3405-9
[PubMed:9642195] [WorldCat.org] (P p)

D L Popham, P Setlow
Cloning, nucleotide sequence, and mutagenesis of the Bacillus subtilis ponA operon, which codes for penicillin-binding protein (PBP) 1 and a PBP-related factor.
J. Bacteriol.: 1995, 177(2);326-35
[PubMed:7814321] [WorldCat.org] [DOI] (P p)