Difference between revisions of "RecJ"

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(References)
(Biological materials)
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* '''Mutant:'''
 
* '''Mutant:'''
 +
** GP895 (''recJ''::''kan''), available in [[Stülke]] lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 10:43, 20 June 2011

  • Description: single-strand DNA-specific exonuclease

Gene name yrvE
Synonyms recJ
Essential no
Product single-strand DNA-specific exonuclease
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 87 kDa, 5.181
Gene length, protein length 2358 bp, 786 aa
Immediate neighbours apt, yrvD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YrvE context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU27620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: recJ family (according to Swiss-Prot)
  • Paralogous protein(s): YorK

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
    • GP895 (recJ::kan), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

M Nickel, G Homuth, C Böhnisch, U Mäder, T Schweder
Cold induction of the Bacillus subtilis bkd operon is mediated by increased mRNA stability.
Mol. Genet. Genomics: 2004, 272(1);98-107
[PubMed:15241682] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J. Bacteriol.: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

V A Sutera, E S Han, L A Rajman, S T Lovett
Mutational analysis of the RecJ exonuclease of Escherichia coli: identification of phosphoesterase motifs.
J. Bacteriol.: 1999, 181(19);6098-102
[PubMed:10498723] [WorldCat.org] (P p)