Difference between revisions of "PyrP"

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* '''Operon:''' ''[[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed]
* '''Operon:''' ''[[pyrR]]-[[pyrP]]-[[pyrB]]-[[pyrC]]-[[pyrAA]]-[[pyrAB]]-[[pyrK]]-[[pyrD]]-[[pyrF]]-[[pyrE]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/8206849 PubMed]
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1709162 PubMed]
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=pyrP_1619023_1620330_1 pyrP] {{PubMed|22383849}}
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/1709162 PubMed]
* '''Regulation:'''  
* '''Regulation:'''  

Revision as of 08:36, 13 April 2012

  • Description: uracil permease

Gene name pyrP
Essential no
Product uracil permease
Function uracil transport in/out via proton symport
Metabolic function and regulation of this protein in SubtiPathways:
Nucleotides (regulation)
MW, pI 45 kDa, 9.504
Gene length, protein length 1302 bp, 434 aa
Immediate neighbours pyrR, pyrB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrP context.gif
This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of nucleotides, membrane proteins

This gene is a member of the following regulons

PyrR regulon

The gene

Basic information

  • Locus tag: BSU15480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: xanthine/uracil permease family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Birgit Hobl, Matthias Mack
The regulator protein PyrR of Bacillus subtilis specifically interacts in vivo with three untranslated regions within pyr mRNA of pyrimidine biosynthesis.
Microbiology (Reading): 2007, 153(Pt 3);693-700
[PubMed:17322189] [WorldCat.org] [DOI] (P p)

Hesheng Zhang, Casper Møller Jørgensen, Robert L Switzer
Mutations affecting transcription pausing in the Bacillus subtilis pyr operon.
Arch Microbiol: 2005, 184(2);101-7
[PubMed:16133632] [WorldCat.org] [DOI] (P p)

Hesheng Zhang, Robert L Switzer
Transcriptional pausing in the Bacillus subtilis pyr operon in vitro: a role in transcriptional attenuation?
J Bacteriol: 2003, 185(16);4764-71
[PubMed:12896995] [WorldCat.org] [DOI] (P p)

Y Lu, R J Turner, R L Switzer
Roles of the three transcriptional attenuators of the Bacillus subtilis pyrimidine biosynthetic operon in the regulation of its expression.
J Bacteriol: 1995, 177(5);1315-25
[PubMed:7868607] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)