Difference between revisions of "PyrD"

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=The gene=
 
=The gene=

Revision as of 08:56, 23 June 2009

  • Description: dihydroorotic acid dehydrogenase (catalytic subunit)

Gene name pyrD
Synonyms pyrDI
Essential no
Product dihydroorotic acid dehydrogenase (catalytic subunit)
Function pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 32 kDa, 4.942
Gene length, protein length 933 bp, 311 aa
Immediate neighbours pyrK, pyrF
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrD context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU15540

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-dihydroorotate + O2 = orotate + H2O2 (according to Swiss-Prot)
  • Protein family: Type 1 subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A E Kahler, F S Nielsen, R L Switzer
Biochemical characterization of the heteromeric Bacillus subtilis dihydroorotate dehydrogenase and its isolated subunits.
Arch. Biochem. Biophys.: 1999, 371(2);191-201
[PubMed:10545205] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J. Bacteriol.: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J. Biol. Chem.: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)