PyrB

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  • Description: aspartate carbamoyltransferase

Gene name pyrB
Synonyms
Essential no
Product aspartate carbamoyltransferase
Function pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 34 kDa, 5.341
Gene length, protein length 912 bp, 304 aa
Immediate neighbours pyrP, pyrC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrB context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU15490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate (according to Swiss-Prot)
  • Protein family: ATCase/OTCase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-303 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • KEGG entry: [3]

Additional information

  • subject to Clp-dependent proteolysis upon glucose starvation PubMed

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information: subject to Clp-dependent proteolysis upon glucose starvation PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol. Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

Hesheng Zhang, Robert L Switzer
Transcriptional pausing in the Bacillus subtilis pyr operon in vitro: a role in transcriptional attenuation?
J. Bacteriol.: 2003, 185(16);4764-71
[PubMed:12896995] [WorldCat.org] [DOI] (P p)

Y Lu, R J Turner, R L Switzer
Roles of the three transcriptional attenuators of the Bacillus subtilis pyrimidine biosynthetic operon in the regulation of its expression.
J. Bacteriol.: 1995, 177(5);1315-25
[PubMed:7868607] [WorldCat.org] [DOI] (P p)

P Hu, R L Switzer
Evidence for substrate stabilization in regulation of the degradation of Bacillus subtilis aspartate transcarbamylase in vivo.
Arch. Biochem. Biophys.: 1995, 316(1);260-6
[PubMed:7840626] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J. Bacteriol.: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J. Biol. Chem.: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)

C G Lerner, R L Switzer
Cloning and structure of the Bacillus subtilis aspartate transcarbamylase gene (pyrB).
J. Biol. Chem.: 1986, 261(24);11156-65
[PubMed:3015959] [WorldCat.org] (P p)

J S Brabson, M R Maurizi, R L Switzer
Aspartate transcarbamylase from Bacillus subtilis.
Meth. Enzymol.: 1985, 113;627-35
[PubMed:3937019] [WorldCat.org] [DOI] (P p)

R W Bond, R L Switzer
Degradation of aspartate transcarbamylase in Bacillus subtilis is deficient in rel mutants but is not mediated by guanosine polyphosphates.
J. Bacteriol.: 1984, 158(2);746-8
[PubMed:6427186] [WorldCat.org] (P p)