PyrAA

From SubtiWiki
Revision as of 08:54, 23 June 2009 by CLammers (talk | contribs)
Jump to: navigation, search
  • Description: carbamoyl-phosphate synthetase (glutaminase subunit)

Gene name pyrAA
Synonyms
Essential no
Product carbamoyl-phosphate synthetase (glutaminase subunit)
Function pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Pyrimidines, Nucleotides (regulation)
MW, pI 39 kDa, 5.818
Gene length, protein length 1092 bp, 364 aa
Immediate neighbours pyrC, pyrAB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PyrAA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU15510

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate (according to Swiss-Prot)
  • Protein family: carA family (according to Swiss-Prot)
  • Paralogous protein(s): CarA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the absence of uridine nucleotides (PyrR) PubMed
  • Regulatory mechanism:
    • PyrR: RNA switch, transcription termination/ antitermination (in the presence of uridine nucleotides: termination, in their absence: antitermination) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J. Bacteriol.: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J. Biol. Chem.: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)