PurA

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  • Description: adenylosuccinate synthetase

Gene name purA
Synonyms
Essential no
Product adenylosuccinate synthetase
Function purine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Purine synthesis, Nucleotides (regulation)
MW, pI 47 kDa, 5.459
Gene length, protein length 1290 bp, 430 aa
Immediate neighbours trnY-Lys, yycE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PurA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU40420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP (according to Swiss-Prot)
  • Protein family: adenylosuccinate synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: expression activated by glucose (4.9 fold) (CcpA) PubMed
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab. Eng.: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] (P p)

M Weng, P L Nagy, H Zalkin
Identification of the Bacillus subtilis pur operon repressor.
Proc. Natl. Acad. Sci. U.S.A.: 1995, 92(16);7455-9
[PubMed:7638212] [WorldCat.org] [DOI] (P p)

H H Saxild, P Nygaard
Regulation of levels of purine biosynthetic enzymes in Bacillus subtilis: effects of changing purine nucleotide pools.
J. Gen. Microbiol.: 1991, 137(10);2387-94
[PubMed:1722815] [WorldCat.org] [DOI] (P p)