PupG

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  • Description: purine nucleoside phosphorylase

Gene name pupG
Synonyms punA, pnp, yqkO
Essential no
Product purine nucleoside phosphorylase
Function purine salvage and interconversion
Gene expression levels in SubtiExpress: pupG
Metabolic function and regulation of this protein in SubtiPathways:
pupG
MW, pI 28 kDa, 4.86
Gene length, protein length 813 bp, 271 aa
Immediate neighbours dacF, drm
Sequences Protein DNA DNA_with_flanks
Genetic context
PunA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PupG expression.png



















Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, phosphoproteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU23490

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate (according to Swiss-Prot) RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate (according to Swiss-Prot)
  • Protein family: PNP/MTAP phosphorylase family (according to Swiss-Prot) polyribonucleotide nucleotidyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on Ser-28 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced in the presence of nucleosides (deoxyribose 5-phosphate and ribose 5-phosphate act as molecular inducers) PubMed
    • subject to carbon catabolite repression (CcpA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References