Difference between revisions of "PucF"

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=The gene=
 
=The gene=

Revision as of 08:49, 23 June 2009

  • Description: allantoate amidohydrolase

Gene name pucF
Synonyms yurH
Essential no
Product allantoate amidohydrolase
Function purine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Purine catabolism, Nucleotides (regulation)
MW, pI 45 kDa, 5.364
Gene length, protein length 1236 bp, 412 aa
Immediate neighbours pucG, yurI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YurH context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU32530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: peptidase M20 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Regulatory mechanism:
    • [PucR]]: transcription activation PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J. Bacteriol.: 2002, 184(12);3232-41
[PubMed:12029039] [WorldCat.org] [DOI] (P p)

A C Schultz, P Nygaard, H H Saxild
Functional analysis of 14 genes that constitute the purine catabolic pathway in Bacillus subtilis and evidence for a novel regulon controlled by the PucR transcription activator.
J. Bacteriol.: 2001, 183(11);3293-302
[PubMed:11344136] [WorldCat.org] [DOI] (P p)