Difference between revisions of "PrkA"

From SubtiWiki
Jump to: navigation, search
m (Phenotypes of a mutant)
m (Phenotypes of a mutant)
Line 49: Line 49:
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
sporulation frequency reduced to 12-27% of wild-type levels [http://www.ncbi.nlm.nih.gov/pubmed/12662922 PubMed]
+
* sporulation frequency reduced to 12-27% of wild-type levels [http://www.ncbi.nlm.nih.gov/pubmed/12662922 PubMed]
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 17:09, 14 October 2014

  • Description: putative serine protein kinase

Gene name prkA
Synonyms
Essential no
Product putative serine protein kinase
Function unknown
Gene expression levels in SubtiExpress: prkA
MW, pI 72 kDa, 5.459
Gene length, protein length 1893 bp, 631 aa
Immediate neighbours yhbF, yhbH
Sequences Protein DNA DNA_with_flanks
Genetic context
PrkA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PrkA expression.png















Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigE regulon

The gene

Basic information

  • Locus tag: BSU08970

Phenotypes of a mutant

  • sporulation frequency reduced to 12-27% of wild-type levels PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: prkA family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 34 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 170 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 22 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 482 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Jihen Tagourti, Ahmed Landoulsi, Gilbert Richarme
Cloning, expression, purification and characterization of the stress kinase YeaG from Escherichia coli.
Protein Expr. Purif.: 2008, 59(1);79-85
[PubMed:18276156] [WorldCat.org] [DOI] (I p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J. Mol. Biol.: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J. Bacteriol.: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

C Fischer, C Geourjon, C Bourson, J Deutscher
Cloning and characterization of the Bacillus subtilis prkA gene encoding a novel serine protein kinase.
Gene: 1996, 168(1);55-60
[PubMed:8626065] [WorldCat.org] [DOI] (P p)