PgpH

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  • Description: c-di-AMP specific phosphodiesterase

Gene name yqfF
Synonyms
Essential no
Product c-di-AMP specific phosphodiesterase
Function control of c-di-AMP homeostasis
Gene expression levels in SubtiExpress: yqfF
MW, pI 78 kDa, 7.697
Gene length, protein length 2133 bp, 711 aa
Immediate neighbours yqfG, phoH
Sequences Protein DNA DNA_with_flanks
Genetic context
YqfF context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqfF expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides, membrane proteins

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU25330

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • hydrolysis of c-di-AMP to 5'-pApA PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:
    • ppGpp acts as allosteric inhibitor of phosphodiesterase activity (in Listeria monocytogenes) PubMed

Database entries

  • Structure:
    • 4S1B (the HD domain of the protein from Listeria monocytogenes in complex with c-di-AMP, 58% identity, 85% similarity) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Expression vector:
  • lacZ fusion: pGP190 (in pAC7), available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Fabian M Commichau, Achim Dickmanns, Jan Gundlach, Ralf Ficner, Jörg Stülke
A jack of all trades: the multiple roles of the unique essential second messenger cyclic di-AMP.
Mol. Microbiol.: 2015, 97(2);189-204
[PubMed:25869574] [WorldCat.org] [DOI] (I p)

John D Helmann
Chemical proteomics reveals a second family of cyclic-di-AMP hydrolases.
Proc. Natl. Acad. Sci. U.S.A.: 2015, 112(7);1921-2
[PubMed:25637595] [WorldCat.org] [DOI] (I p)

Original publications

TuAnh Ngoc Huynh, Shukun Luo, Daniel Pensinger, John-Demian Sauer, Liang Tong, Joshua J Woodward
An HD-domain phosphodiesterase mediates cooperative hydrolysis of c-di-AMP to affect bacterial growth and virulence.
Proc. Natl. Acad. Sci. U.S.A.: 2015, 112(7);E747-56
[PubMed:25583510] [WorldCat.org] [DOI] (I p)

Elodie Marchadier, Rut Carballido-López, Sophie Brinster, Céline Fabret, Peggy Mervelet, Philippe Bessières, Marie-Françoise Noirot-Gros, Vincent Fromion, Philippe Noirot
An expanded protein-protein interaction network in Bacillus subtilis reveals a group of hubs: Exploration by an integrative approach.
Proteomics: 2011, 11(15);2981-91
[PubMed:21630458] [WorldCat.org] [DOI] (I p)