Difference between revisions of "PckA"

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=Expression and regulation=
=Expression and regulation=
* '''Operon:''' ''[[pckA]]'' {{PubMed|9387221}}
* '''Operon:'''  
* '''Sigma factor:''' [[SigA]] {{PubMed|15720552}}
* '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+15720552 PubMed]
* '''Regulation:'''  
** repressed by glucose (35-fold) [[CcpN]] {{PubMed|15720552,12850135}}
* '''Regulation:''' repressed by glucose (7-fold) [http://www.ncbi.nlm.nih.gov/pubmed/12850135 PubMed],  repressed (35-times) Glc, repressor [[CcpN]] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+15720552 PubMed]
* '''Regulatory mechanism:'''  
** [[CcpN]]: transcription repression {{PubMed|15720552}}
* '''Regulatory mechanism:''' transcription repression
* '''Additional information:'''
* '''Additional information:'''
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<pubmed>12850135 15720552 18586936, </pubmed>
<pubmed>12850135 15720552 18586936, 9387221 </pubmed>
[[Category:Protein-coding genes]]
[[Category:Protein-coding genes]]

Revision as of 20:21, 19 December 2009

  • Description: phosphoenolpyruvate carboxykinase

Gene name pckA
Synonyms ppc
Essential no
Product phosphoenolpyruvate carboxykinase
Function synthesis of phosphoenolpyruvate
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 58,1 kDa, 5.12
Gene length, protein length 1581 bp, 527 amino acids
Immediate neighbours metK, ytmB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PckA context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU30560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
  • Protein family: phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Nucleotide binding Domain (233–240)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

  • Structure: 2PXZ (E.coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks


Simon Tännler, Eliane Fischer, Dominique Le Coq, Thierry Doan, Emmanuel Jamet, Uwe Sauer, Stéphane Aymerich
CcpN controls central carbon fluxes in Bacillus subtilis.
J. Bacteriol.: 2008, 190(18);6178-87
[PubMed:18586936] [WorldCat.org] [DOI] (I p)

Pascale Servant, Dominique Le Coq, Stéphane Aymerich
CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of Bacillus subtilis gluconeogenic genes.
Mol. Microbiol.: 2005, 55(5);1435-51
[PubMed:15720552] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab. Eng.: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

A Lapidus, N Galleron, A Sorokin, S D Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading, Engl.): 1997, 143 ( Pt 11);3431-41
[PubMed:9387221] [WorldCat.org] [DOI] (P p)