Difference between revisions of "PckA"

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(Biological materials)
(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
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** pGP1753 (N-terminal Strep-tag, for [[SPINE]], purification from ''B. subtilis'', in [[pGP380]]) (available in [[Stülke]] lab)
 
 
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 18:37, 11 May 2010

  • Description: phosphoenolpyruvate carboxykinase

Gene name pckA
Synonyms ppc
Essential no
Product phosphoenolpyruvate carboxykinase
Function synthesis of phosphoenolpyruvate
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 58,1 kDa, 5.12
Gene length, protein length 1581 bp, 527 amino acids
Immediate neighbours metK, ytmB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PckA context.gif
This image was kindly provided by SubtiList








The gene

Basic information

  • Locus tag: BSU30560

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2 (according to Swiss-Prot) ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO(2)
  • Protein family: phosphoenolpyruvate carboxykinase [ATP] family (according to Swiss-Prot) phosphoenolpyruvate carboxykinase [ATP] family
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
    • Nucleotide binding Domain (233–240)
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot), cytoplasm

Database entries

  • Structure: 2PXZ (E.coli)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
    • pGP1753 (N-terminal Strep-tag, for SPINE, purification from B. subtilis, in pGP380) (available in Stülke lab)
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References