PbuO

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  • Description: similar to hypoxanthine/guanine permease PbuG

Gene name pbuO
Synonyms ytiP
Essential no
Product unknown
Function unknown
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 45 kDa, 7.484
Gene length, protein length 1296 bp, 432 aa
Immediate neighbours ytjP, ythQ
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YtiP context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU29990

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): PbuG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
  • Regulatory mechanism:
    • PurR: transcription repression PubMed
    • PurR: transcription repression (molecular inducer: PRPP) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J. Bacteriol.: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)