PbuG

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  • Description: hypoxanthin/ guanine permease

Gene name pbuG
Synonyms yebB
Essential no
Product hypoxanthin/ guanine permease
Function hypoxanthine and guanine uptake
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 46 kDa, 8.808
Gene length, protein length 1320 bp, 440 aa
Immediate neighbours guaA, yebC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PbuG context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU06370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): PbuO

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: membrane PubMed

Database entries

  • Structure: 2EEW (the pbuG riboswitch, U47c mutant, bound to hypoxanthine)
  • Swiss prot entry:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon: pbuG
  • Regulation:
    • repressed in the presence of purine nucleotides (PurR) PubMed
    • repressed by hypoxanthine and guanine, PurR-independent, (G-box)PubMed
    • repressed in the presence of adenine or adenosine(PurR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Lars Engholm Johansen, Per Nygaard, Catharina Lassen, Yvonne Agersø, Hans H Saxild
Definition of a second Bacillus subtilis pur regulon comprising the pur and xpt-pbuX operons plus pbuG, nupG (yxjA), and pbuE (ydhL).
J. Bacteriol.: 2003, 185(17);5200-9
[PubMed:12923093] [WorldCat.org] [DOI] (P p)

H H Saxild, K Brunstedt, K I Nielsen, H Jarmer, P Nygaard
Definition of the Bacillus subtilis PurR operator using genetic and bioinformatic tools and expansion of the PurR regulon with glyA, guaC, pbuG, xpt-pbuX, yqhZ-folD, and pbuO.
J. Bacteriol.: 2001, 183(21);6175-83
[PubMed:11591660] [WorldCat.org] [DOI] (P p)