Difference between revisions of "PTTB2"

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The vector pTTB2 is a high copy '''food grade''' expression vector for constitutive expression. Selection is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded).  For easier handling pTTB2 is designed as ''B. subtilis / E. coli'' shuttle vector. The parts of the vector used for cloning with ''E. coli'' (''E. coli'' origin and ampicillin resistance cassette) can be eliminated afterwards by restriction enzyme cleavage and religation of the vector. This technique connects the advantage of easy cloning with ''E. coli'' with the food grade property of ''B. subtilis''
 
The vector pTTB2 is a high copy '''food grade''' expression vector for constitutive expression. Selection is based on the interplay of the endogenous ''Bacillus'' toxin EndoA (encoded within the genome of host strains ''B. subtilis'' TEA or ''B. subtilis'' WEA) and its antitoxin EndoB (vector encoded).  For easier handling pTTB2 is designed as ''B. subtilis / E. coli'' shuttle vector. The parts of the vector used for cloning with ''E. coli'' (''E. coli'' origin and ampicillin resistance cassette) can be eliminated afterwards by restriction enzyme cleavage and religation of the vector. This technique connects the advantage of easy cloning with ''E. coli'' with the food grade property of ''B. subtilis''

Revision as of 10:26, 28 August 2017

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PTTB2.PNG PTTB2-Legende.jpg

The vector pTTB2 is a high copy food grade expression vector for constitutive expression. Selection is based on the interplay of the endogenous Bacillus toxin EndoA (encoded within the genome of host strains B. subtilis TEA or B. subtilis WEA) and its antitoxin EndoB (vector encoded). For easier handling pTTB2 is designed as B. subtilis / E. coli shuttle vector. The parts of the vector used for cloning with E. coli (E. coli origin and ampicillin resistance cassette) can be eliminated afterwards by restriction enzyme cleavage and religation of the vector. This technique connects the advantage of easy cloning with E. coli with the food grade property of B. subtilis

Features

  • Food grade protein production
  • Stable high level expression without addition of any antibiotics
  • All DNA contained in the final expression system is derived from B. subtilis
  • No endotoxins are produced
  • Corresponding protease-deficient strain for secretory protein production is available

The pTTB2 vector and corresponding strains B. subtilis TEA and B. subtilis WEA are available from MoBiTec GmbH (Göttingen)

References

Sen Yang, Zhen Kang, Wenlong Cao, Guocheng Du, Jian Chen
Construction of a novel, stable, food-grade expression system by engineering the endogenous toxin-antitoxin system in Bacillus subtilis.
J. Biotechnol.: 2016, 219;40-7
[PubMed:26721182] [WorldCat.org] [DOI] (I p)