The pHT43 vector is a high-level expression vector for recombinant protein production and secretion into the culture medium. The expression is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. The amyQ signal sequence (encoding for the signal peptide of a-amylase) is located downstream of the Shine-Dalgarno Sequence, followed by restriction sites for cloning the gene of interest. pHT43 is an E. coli / B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.
- Secretory protein production
- Efficiently controllable gene expression (IPTG inducible)
- High level production and secretion of recombinant proteins
- E. coli / B. subtilis shuttle vector
pHT43 and the eight-fold protease-deficient B. subtilis strain WB800N, for secretory protein production are available from MoBiTec GmbH (Göttingen)