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The pHT01 is a high-level intracellular expression vector for recombinant protein production with B. subtilis. The vector is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.


  • Efficiently controllable gene expression (IPTG inducible)
  • High level production of recombinant proteins
  • E. coli / B. subtilis shuttle vector