The content of this page/section is provided by a third party. SubtiWiki acknowledges that this third party content is obtained from a source believed to be reliable. Please note that no guarantees are made by SubtiWiki as to reliability of this third party information. Provision of this third party content is for general information purpose and does not constitute a recommendation. SubtiWiki does not own copyright over this third party content (including but not limited to text, images, and media).
The pHT01 is a high-level intracellular expression vector for recombinant protein production with B. subtilis. The vector is based on the strong σA-dependent promoter preceding the groE operon of B. subtilis which has been converted into an efficiently controllable (IPTG-inducible) promoter by addition of the lac operator. pHT01 is an E. coli/ B. subtilis shuttle vector, that provides ampicillin resistance to E.coli and chloramphenicol resistance to B. subtilis.