PGP574

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Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP172

Oliver Schilling, Christina Herzberg, Tina Hertrich, Hanna Vörsmann, Dirk Jessen, Sebastian Hübner, Fritz Titgemeyer, Jörg Stülke
Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples.
Nucleic Acids Res.: 2006, 34(21);6102-15
[PubMed:17074746] [WorldCat.org] [DOI] (I p)


  • sequencing primer:
    • T7: 5’-TAATACGACTCACTATAGGG -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’