Difference between revisions of "PGP574"

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similar [[plasmid]]: [[pGP172]]
 
similar [[plasmid]]: [[pGP172]]
 
<pubmed> 17074746 </pubmed>
 
  
 
* sequencing primer:  
 
* sequencing primer:  
 
**'''T7:''' 5’-TAATACGACTCACTATAGGG -3’
 
**'''T7:''' 5’-TAATACGACTCACTATAGGG -3’
 
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 +
 +
<pubmed> 17074746 </pubmed>

Revision as of 09:11, 18 January 2012

Vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the C-terminus of the expressed protein

host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture

constructed in the Stülke lab

in E. coli: ampicillin resistance

based on pET3C

similar plasmid: pGP172

  • sequencing primer:
    • T7: 5’-TAATACGACTCACTATAGGG -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Oliver Schilling, Christina Herzberg, Tina Hertrich, Hanna Vörsmann, Dirk Jessen, Sebastian Hübner, Fritz Titgemeyer, Jörg Stülke
Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples.
Nucleic Acids Res.: 2006, 34(21);6102-15
[PubMed:17074746] [WorldCat.org] [DOI] (I p)