Difference between revisions of "PGP172"

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* vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
+
* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
  
* host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
+
* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  
 
* constructed in the [[Stülke]] lab
 
* constructed in the [[Stülke]] lab
  
* in E. coli: ampicillin resistance
+
* in ''E. coli'': ampicillin resistance
  
 
* based on pET3C
 
* based on pET3C

Revision as of 08:13, 30 July 2010

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  • in E. coli: ampicillin resistance
  • based on pET3C
  • sequencing primer:
    • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur. J. Biochem.: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)