Difference between revisions of "PGP172"

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(Undo revision 121171 by Fabian Commichau (Talk))
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vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
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* vector for the expression of proteins in E. coli, the [[plasmid]] allows to fuse a Strep-tag to the N-terminus of the expressed protein
  
host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
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* host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  
constructed in the [[Stülke]] lab
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* constructed in the [[Stülke]] lab
  
in E. coli: ampicillin resistance
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* in E. coli: ampicillin resistance
  
based on pET3C
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* based on pET3C
  
similar [[plasmid]]: [[pGP574]]
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* similar [[plasmid]]: [[pGP574]]
  
sequencing primer:  
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* sequencing primer:  
*'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
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**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
*'''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
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** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  
 
<pubmed> 14717704 </pubmed>
 
<pubmed> 14717704 </pubmed>

Revision as of 11:19, 14 April 2010

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  • in E. coli: ampicillin resistance
  • based on pET3C
  • sequencing primer:
    • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’

Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur. J. Biochem.: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)