Difference between revisions of "PGP172"

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**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
**'''CD13:''' 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
 
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 
** '''NP20:''' 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
 +
 +
* important: EcoRI, although mentioned in the MCS, is not unique!
 +
  
 
<pubmed> 14717704 </pubmed>
 
<pubmed> 14717704 </pubmed>

Revision as of 07:55, 16 April 2013

pGP172: click to enlarge
pGP172 cloning region: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a Strep-tag to the N-terminus of the expressed protein
  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  • in E. coli: ampicillin resistance
  • based on pET3C
  • sequencing primer:
    • CD13: 5’-AAACATATGGCTAGCTGGAGCCACCCGCAGTTC -3’
    • NP20: 5’-GCAGCAGCCAACTCAGCTTCCTTTCGGGC-3’
  • important: EcoRI, although mentioned in the MCS, is not unique!


Matthias Merzbacher, Christian Detsch, Wolfgang Hillen, Jörg Stülke
Mycoplasma pneumoniae HPr kinase/phosphorylase.
Eur. J. Biochem.: 2004, 271(2);367-74
[PubMed:14717704] [WorldCat.org] [DOI] (P p)