Difference between revisions of "PETM-11"

From SubtiWiki
Jump to: navigation, search
Line 4: Line 4:
  
 
* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
 
* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
 +
  
 
* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 
* host: ''E. coli'' BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
 +
  
 
* in ''E. coli'': ampicillin resistance
 
* in ''E. coli'': ampicillin resistance
 +
  
 
*from EMBL
 
*from EMBL
 +
  
 
*sequencing primer:  
 
*sequencing primer:  

Revision as of 10:55, 19 November 2015

pWH844: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease


  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture


  • in E. coli: ampicillin resistance


  • from EMBL


  • sequencing primer:
  • CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
  • CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘



Annett Dümmler, Ann-Marie Lawrence, Ario de Marco
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.
Microb. Cell Fact.: 2005, 4;34
[PubMed:16351710] [WorldCat.org] [DOI] (I e)

Return to Plasmids