Difference between revisions of "PETM-11"

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[[File:pETM-11.png|250px|thumb|right|'''pWH844: click to enlarge''']]
 
[[File:pETM-11.png|250px|thumb|right|'''pWH844: click to enlarge''']]
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* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
 
* vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
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* in ''E. coli'': ampicillin resistance
 
* in ''E. coli'': ampicillin resistance
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*from EMBL
  
 
*sequencing primer:  
 
*sequencing primer:  
 
*'''CZ103'''</font>: 5’-GAGGATCGAGATCTCGATC-3‘
 
*'''CZ103'''</font>: 5’-GAGGATCGAGATCTCGATC-3‘
 
*'''CZ104''': 5’-CTAGTTATTGCTCAGCGGTG-3‘
 
*'''CZ104''': 5’-CTAGTTATTGCTCAGCGGTG-3‘
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<pubmed> 16351710 </pubmed>
 
<pubmed> 16351710 </pubmed>
 
==  Return to [[Plasmids]] ==
 
==  Return to [[Plasmids]] ==

Revision as of 10:54, 19 November 2015

pWH844: click to enlarge


  • vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein that can be cleaved of by the TEV protease
  • host: E. coli BL21(DE3)/pLysS, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
  • in E. coli: ampicillin resistance
  • from EMBL
  • sequencing primer:
  • CZ103</font>: 5’-GAGGATCGAGATCTCGATC-3‘
  • CZ104: 5’-CTAGTTATTGCTCAGCGGTG-3‘



Annett Dümmler, Ann-Marie Lawrence, Ario de Marco
Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors.
Microb. Cell Fact.: 2005, 4;34
[PubMed:16351710] [WorldCat.org] [DOI] (I e)

Return to Plasmids