Difference between revisions of "PAC6"

From SubtiWiki
Jump to: navigation, search
Line 9: Line 9:
in ''E. coli'': ampicillin resistance
in ''E. coli'': ampicillin resistance
in ''B. subtilis'': chloramphenocol resistance
in ''B. subtilis'': chloramphenicol resistance
similar vectors: [[pAC5]], [[pAC7]]
similar vectors: [[pAC5]], [[pAC7]]

Revision as of 15:40, 22 September 2011

pAC6: click to enlarge

vector for the construction of transcriptional lacZ fusions that can be integrated at the amyE site

does not replicate in B. subtilis, integrates into the amyE site

constructed in the Rapoport lab

in E. coli: ampicillin resistance

in B. subtilis: chloramphenicol resistance

similar vectors: pAC5, pAC7

sequencing primer:


J Stülke, I Martin-Verstraete, M Zagorec, M Rose, A Klier, G Rapoport
Induction of the Bacillus subtilis ptsGHI operon by glucose is controlled by a novel antiterminator, GlcT.
Mol. Microbiol.: 1997, 25(1);65-78
[PubMed:11902727] [WorldCat.org] (P p)