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  • Description: nitrogen-regulated PII-like protein

Gene name nrgB
Synonyms glnK
Essential no
Product nitrogen-regulated PII-like protein
Function regulation of ammonium uptake
Gene expression levels in SubtiExpress: nrgB
Interactions involving this protein in SubtInteract: NrgB
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 12 kDa, 8.825
Gene length, protein length 348 bp, 116 aa
Immediate neighbours nrgA, bcrC
Sequences Protein DNA DNA_with_flanks
Genetic context
NrgB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
NrgB expression.png

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, glutamate metabolism, transcription factors and their control, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

  • Locus tag: BSU36520

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: P(II) protein family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induced by ammonium limitation
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP259 (cat), GP255 (nrgAB, cat), both available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:

Labs working on this gene/protein

Susan Fisher, Boston, USA homepage

Your additional remarks



Karl Forchhammer
P(II) signal transducers: novel functional and structural insights.
Trends Microbiol.: 2008, 16(2);65-72
[PubMed:18182294] [] [DOI] (P p)

T Arcondéguy, R Jack, M Merrick
P(II) signal transduction proteins, pivotal players in microbial nitrogen control.
Microbiol. Mol. Biol. Rev.: 2001, 65(1);80-105
[PubMed:11238986] [] [DOI] (P p)

G Thomas, G Coutts, M Merrick
The glnKamtB operon. A conserved gene pair in prokaryotes.
Trends Genet.: 2000, 16(1);11-4
[PubMed:10637624] [] (P p)

Original publications

Martha V Radchenko, Jeremy Thornton, Mike Merrick
P(II) signal transduction proteins are ATPases whose activity is regulated by 2-oxoglutarate.
Proc. Natl. Acad. Sci. U.S.A.: 2013, 110(32);12948-53
[PubMed:23818625] [] [DOI] (I p)

Ksenia Fedorova, Airat Kayumov, Kathrin Woyda, Olga Ilinskaja, Karl Forchhammer
Transcription factor TnrA inhibits the biosynthetic activity of glutamine synthetase in Bacillus subtilis.
FEBS Lett.: 2013, 587(9);1293-8
[PubMed:23535029] [] [DOI] (I p)

Minsu Kim, Zhongge Zhang, Hiroyuki Okano, Dalai Yan, Alexander Groisman, Terence Hwa
Need-based activation of ammonium uptake in Escherichia coli.
Mol. Syst. Biol.: 2012, 8;616
[PubMed:23010999] [] [DOI] (I p)

Airat Kayumov, Annette Heinrich, Kseniya Fedorova, Olga Ilinskaya, Karl Forchhammer
Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis.
FEBS J.: 2011, 278(10);1779-89
[PubMed:21435182] [] [DOI] (I p)

Matthew J Conroy, Anne Durand, Domenico Lupo, Xiao-Dan Li, Per A Bullough, Fritz K Winkler, Mike Merrick
The crystal structure of the Escherichia coli AmtB-GlnK complex reveals how GlnK regulates the ammonia channel.
Proc. Natl. Acad. Sci. U.S.A.: 2007, 104(4);1213-8
[PubMed:17220269] [] [DOI] (P p)

Annette Heinrich, Kathrin Woyda, Katja Brauburger, Gregor Meiss, Christian Detsch, Jörg Stülke, Karl Forchhammer
Interaction of the membrane-bound GlnK-AmtB complex with the master regulator of nitrogen metabolism TnrA in Bacillus subtilis.
J. Biol. Chem.: 2006, 281(46);34909-17
[PubMed:17001076] [] [DOI] (P p)

Christian Detsch, Jörg Stülke
Ammonium utilization in Bacillus subtilis: transport and regulatory functions of NrgA and NrgB.
Microbiology (Reading, Engl.): 2003, 149(Pt 11);3289-97
[PubMed:14600241] [] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol. Microbiol.: 2003, 49(1);157-65
[PubMed:12823818] [] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc. Natl. Acad. Sci. U.S.A.: 1996, 93(17);8841-5
[PubMed:8799114] [] [DOI] (P p)

L V Wray, M R Atkinson, S H Fisher
The nitrogen-regulated Bacillus subtilis nrgAB operon encodes a membrane protein and a protein highly similar to the Escherichia coli glnB-encoded PII protein.
J. Bacteriol.: 1994, 176(1);108-14
[PubMed:8282685] [] [DOI] (P p)

M R Atkinson, S H Fisher
Identification of genes and gene products whose expression is activated during nitrogen-limited growth in Bacillus subtilis.
J. Bacteriol.: 1991, 173(1);23-7
[PubMed:1670935] [] [DOI] (P p)