Difference between revisions of "NasC"

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(Expression and regulation)
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=Expression and regulation=
=Expression and regulation=
* '''Operon:'''  
* '''Operon:''' ''[[nasB]]-[[nasC]]-[[nasD]]-[[nasE]]-[[nasF]]'' {{PubMed|7836289}}
* '''[[Sigma factor]]:'''  
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|7836289}}
* '''Regulation:'''  
* '''Regulation:'''  
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** [[NsrR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
** [[NsrR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/16885456 PubMed]
* '''Additional information:'''  
* '''Additional information:'''
=Biological materials =
=Biological materials =

Revision as of 20:26, 5 August 2009

  • Description: nitrate reductase (catalytic subunit)

Gene name nasC
Synonyms narB, nasBB
Essential no
Product nitrate reductase (catalytic subunit)
Function nitrate respiration
MW, pI 78 kDa, 6.728
Gene length, protein length 2130 bp, 710 aa
Immediate neighbours nasD, nasB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NasC context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU03310

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nitrite + acceptor = nitrate + reduced acceptor (according to Swiss-Prot)
  • Protein family: prokaryotic molybdopterin-containing oxidoreductase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed unless NO is present under anaerobic conditions (NsrR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Michiko M Nakano, Hao Geng, Shunji Nakano, Kazuo Kobayashi
The nitric oxide-responsive regulator NsrR controls ResDE-dependent gene expression.
J Bacteriol: 2006, 188(16);5878-87
[PubMed:16885456] [WorldCat.org] [DOI] (P p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

L V Wray, A E Ferson, K Rohrer, S H Fisher
TnrA, a transcription factor required for global nitrogen regulation in Bacillus subtilis.
Proc Natl Acad Sci U S A: 1996, 93(17);8841-5
[PubMed:8799114] [WorldCat.org] [DOI] (P p)