MotA

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  • Description: H+-coupled MotA-MotB flagellar stator

Gene name motA
Synonyms mot
Essential no
Product motility protein (flagellar motor rotation)
Function motility and chemotaxis
Gene expression levels in SubtiExpress: motA
Interactions involving this protein in SubtInteract: MotA
MW, pI 29 kDa, 5.01
Gene length, protein length 810 bp, 270 aa
Immediate neighbours motB, clpE
Sequences Protein DNA DNA_with_flanks
Genetic context
MotA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MotA expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

SigD regulon

The gene

Basic information

  • Locus tag: BSU13690

Phenotypes of a mutant

  • loss of swimming and swarming motility PubMed
  • mucoid phenotype due to the DegU-P activated overexpression of the capB-capC-capA-capE operon and resulting overproduction of poly-gamma-glutamate PubMed
  • not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: motA family (according to Swiss-Prot)
  • Paralogous protein(s): MotP

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 100 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 613 PubMed

Biological materials

  • Mutant:
    • 1A631 ( motA::erm), PubMed, available at BGSC
    • 1A923 ( motA::erm), PubMed, available at BGSC
    • DS7498 (marker-less in NCIB3610) PubMed
    • TB193 amyE::Phy-sfgfp (marker-less in NCIB3610 with constitutive expressed sfgfp) PubMed
    • TB210 amyE::Phy-mKATE2 (marker-less in NCIB3610 with constitutive expressed mKATE2) PubMed
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Theresa Hölscher, Benjamin Bartels, Yu-Cheng Lin, Ramses Gallegos-Monterrosa, Alexa Price-Whelan, Roberto Kolter, Lars E P Dietrich, Ákos T Kovács
Motility, Chemotaxis and Aerotaxis Contribute to Competitiveness during Bacterial Pellicle Biofilm Development.
J. Mol. Biol.: 2015, 427(23);3695-3708
[PubMed:26122431] [WorldCat.org] [DOI] (I p)

Yuka Takahashi, Masahiro Ito
Mutational analysis of charged residues in the cytoplasmic loops of MotA and MotP in the Bacillus subtilis flagellar motor.
J. Biochem.: 2014, 156(4);211-20
[PubMed:24771657] [WorldCat.org] [DOI] (I p)

Jia Mun Chan, Sarah B Guttenplan, Daniel B Kearns
Defects in the flagellar motor increase synthesis of poly-γ-glutamate in Bacillus subtilis.
J. Bacteriol.: 2014, 196(4);740-53
[PubMed:24296669] [WorldCat.org] [DOI] (I p)

Yun Chen, Yunrong Chai, Jian-hua Guo, Richard Losick
Evidence for cyclic Di-GMP-mediated signaling in Bacillus subtilis.
J. Bacteriol.: 2012, 194(18);5080-90
[PubMed:22821967] [WorldCat.org] [DOI] (I p)

Christopher T Brown, Laura K Fishwick, Binna M Chokshi, Marissa A Cuff, Jay M Jackson, Travis Oglesby, Alison T Rioux, Enrique Rodriguez, Gregory S Stupp, Austin H Trupp, James S Woollcombe-Clarke, Tracy N Wright, William J Zaragoza, Jennifer C Drew, Eric W Triplett, Wayne L Nicholson
Whole-genome sequencing and phenotypic analysis of Bacillus subtilis mutants following evolution under conditions of relaxed selection for sporulation.
Appl. Environ. Microbiol.: 2011, 77(19);6867-77
[PubMed:21821766] [WorldCat.org] [DOI] (I p)

M Z Gilman, M J Chamberlin
Developmental and genetic regulation of Bacillus subtilis genes transcribed by sigma 28-RNA polymerase.
Cell: 1983, 35(1);285-93
[PubMed:6313226] [WorldCat.org] [DOI] (P p)