Difference between revisions of "MleA"

From SubtiWiki
Jump to: navigation, search
Line 24: Line 24:
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqkK]]'', ''[[mleN]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yqkK]]'', ''[[mleN]]''
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23550 Advanced_DNA]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU23550 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU23550 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU23550 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mleA_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:mleA_context.gif]]

Revision as of 11:38, 14 May 2013

  • Description: malic enzyme

Gene name mleA
Synonyms yqkJ
Essential no
Product NAD-dependent malate dehydrogenase
Function malate utilization
Gene expression levels in SubtiExpress: mleA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 45 kDa, 4.895
Gene length, protein length 1317 bp, 439 aa
Immediate neighbours yqkK, mleN
Sequences Protein DNA DNA_with_flanks
Genetic context
MleA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
MleA expression.png



















Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

AnsR regulon, CcpA regulon

The gene

Basic information

  • Locus tag: BSU23550

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): YtsJ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant: GP1136 (cat) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed

Frederik M Meyer, Jörg Stülke
Malate metabolism in Bacillus subtilis: distinct roles for three classes of malate-oxidizing enzymes.
FEMS Microbiol. Lett.: 2013, 339(1);17-22
[PubMed:23136871] [WorldCat.org] [DOI] (I p)

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J. Bacteriol.: 2006, 188(13);4727-36
[PubMed:16788182] [WorldCat.org] [DOI] (P p)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J. Bacteriol.: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)