Difference between revisions of "MleA"

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(References)
(Biological materials)
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=Biological materials =
 
=Biological materials =
  
* '''Mutant:'''
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* '''Mutant:''' GP1136 (cat) available in [[Stülke]] lab
 +
 
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 17:06, 16 August 2010

  • Description: malic enzyme

Gene name mleA
Synonyms yqkJ
Essential no
Product NAD-dependent malate dehydrogenase
Function malate utilization
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 45 kDa, 4.895
Gene length, protein length 1317 bp, 439 aa
Immediate neighbours yqkK, mleN
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MleA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU23550

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): YtsJ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1136 (cat) available in Stülke lab


  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References