MiaA

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  • Description: tRNA isopentenylpyrophosphate transferase

Gene name miaA
Synonyms
Essential no
Product tRNA isopentenylpyrophosphate transferase
Function tRNA modification
MW, pI 35 kDa, 6.002
Gene length, protein length 942 bp, 314 aa
Immediate neighbours ymaF, hfq
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MiaA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU17330

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine (according to Swiss-Prot)
  • Protein family: IPP transferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

The corresponding gene/ protein in other bacteria

J Zhao, H E Leung, M E Winkler
The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions.
J. Bacteriol.: 2001, 183(5);1796-800
[PubMed:11160115] [WorldCat.org] [DOI] (P p)

J M Durand, G R Björk, A Kuwae, M Yoshikawa, C Sasakawa
The modified nucleoside 2-methylthio-N6-isopentenyladenosine in tRNA of Shigella flexneri is required for expression of virulence genes.
J. Bacteriol.: 1997, 179(18);5777-82
[PubMed:9294434] [WorldCat.org] [DOI] (P p)

J Caillet, L Droogmans
Molecular cloning of the Escherichia coli miaA gene involved in the formation of delta 2-isopentenyl adenosine in tRNA.
J. Bacteriol.: 1988, 170(9);4147-52
[PubMed:3045085] [WorldCat.org] [DOI] (P p)