Difference between revisions of "McsB"

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(Biological materials)
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* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
 
* '''Expression vector:''' for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
 +
** expression of native ''mcsB'' in ''B. subtilis'': pBP529186 (in [[pBQ200]]), available in [[Fabian Commichau]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 13:08, 21 July 2015

Gene name mcsB
Synonyms yacI
Essential no
Product protein arginine kinase
Function control of CtsR activity
Gene expression levels in SubtiExpress: mcsB
Interactions involving this protein in SubtInteract: McsB
Metabolic function and regulation of this protein in SubtiPathways:
mcsB
MW, pI 40 kDa, 5.068
Gene length, protein length 1089 bp, 363 aa
Immediate neighbours mcsA, clpC
Sequences Protein DNA DNA_with_flanks
Genetic context
McsB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
McsB expression.png















Categories containing this gene/protein

protein modification, transcription factors and their control, sporulation proteins, general stress proteins (controlled by SigB), heat shock proteins

This gene is a member of the following regulons

CtsR regulon, SigB regulon, SigF regulon

The gene

Basic information

  • Locus tag: BSU00850

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: targets non-functional CtsR for degradation by ClpP/ClpC PubMed
  • Protein family: ATP:guanido phosphotransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
    • autophosphorylation on specific arginine residues PubMed, dephosphorylation by YwlE PubMed
    • autophosphorylation stimulates McsB activity as adaptor protein for ClpC PubMed
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 142 PubMed

Biological materials

  • Mutant:
    • mcsB::aphA3 availbale from the Gerth lab
    • mcsBC167S::spec available from the Gerth lab
    • GP1457 (mcsB::aphA3), available in Jörg Stülke's lab
    • BP69 (spc), available in Fabian Commichau's lab
  • Expression vector: for expression, purification in E. coli with N-terminal His-tag, pRSETA available in Gerth lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody: available in Gerth lab

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original Publications