Difference between revisions of "MalS"

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(Biological materials)
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[utilization of specific carbon sources]]}}
 
=The protein=
 
=The protein=
  

Revision as of 18:49, 30 November 2010

  • Description: malate dehydrogenase (decarboxylating)

Gene name malS
Synonyms
Essential no
Product malate dehydrogenase (decarboxylating)
Function malate utilization
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 61 kDa, 4.895
Gene length, protein length 1698 bp, 566 aa
Immediate neighbours ytzB, ytnP
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
MalS context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU29880

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

Categories containing this gene/protein

utilization of specific carbon sources

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1142 (spc) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J. Bacteriol.: 2006, 188(13);4727-36
[PubMed:16788182] [WorldCat.org] [DOI] (P p)

A Lapidus, N Galleron, A Sorokin, S D Ehrlich
Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.
Microbiology (Reading, Engl.): 1997, 143 ( Pt 11);3431-41
[PubMed:9387221] [WorldCat.org] [DOI] (P p)