Difference between revisions of "Main Page"

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<big>'''Paper of the month: June 2015'''</big>  
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<big>'''Paper of the month: July 2015'''</big>  
 
* The precise functions of serine [[Protein kinases and phosphatases|protein kinases]] in ''B. subtilis'' have largely remained enigmatic until very recently. Now, two studies from the lab of [[Jonathan Dworkin]] describe functions and molecular targets for two of these kinases, [[PrkC]] and [[YabT]]. [http://www.ncbi.nlm.nih.gov/pubmed/26056311 Pereira et al.] have identified the universally conserved [[TufA|elongation factor Tu]] as a target for the protein kinase [[YabT]]. In srarving cells, YabT phosphorylates [[TufA|EF-Tu]] at a conserved threonine residue. Phosphorylation impairs the essential GTPase activity of [[TufA|EF-Tu]], thereby preventing its release from the [[ribosome]]. As a consequence, phosphorylated [[TufA|EF-Tu]] has a dominant-negative effect in [[translation]] elongation, resulting in the overall inhibition of protein synthesis. Importantly, this mechanism allows a quick and robust regulation of one of the [[most abundant proteins|most abundant cellular proteins]]. [http://www.ncbi.nlm.nih.gov/pubmed/26102633 Libby et al.] have uncovered that phosphorylation by PrkC stimulates the activity of the [[essential genes|essential]] [[Two-component systems|two-component transcription factor]] [[WalR]]. This mechanism links the presence of muropeptides that trigger [[PrkC]] activity to the expression of the genes of the [[WalR regulon]] that are involved in [[cell wall synthesis|cell wall metabolism]].
 
* The precise functions of serine [[Protein kinases and phosphatases|protein kinases]] in ''B. subtilis'' have largely remained enigmatic until very recently. Now, two studies from the lab of [[Jonathan Dworkin]] describe functions and molecular targets for two of these kinases, [[PrkC]] and [[YabT]]. [http://www.ncbi.nlm.nih.gov/pubmed/26056311 Pereira et al.] have identified the universally conserved [[TufA|elongation factor Tu]] as a target for the protein kinase [[YabT]]. In srarving cells, YabT phosphorylates [[TufA|EF-Tu]] at a conserved threonine residue. Phosphorylation impairs the essential GTPase activity of [[TufA|EF-Tu]], thereby preventing its release from the [[ribosome]]. As a consequence, phosphorylated [[TufA|EF-Tu]] has a dominant-negative effect in [[translation]] elongation, resulting in the overall inhibition of protein synthesis. Importantly, this mechanism allows a quick and robust regulation of one of the [[most abundant proteins|most abundant cellular proteins]]. [http://www.ncbi.nlm.nih.gov/pubmed/26102633 Libby et al.] have uncovered that phosphorylation by PrkC stimulates the activity of the [[essential genes|essential]] [[Two-component systems|two-component transcription factor]] [[WalR]]. This mechanism links the presence of muropeptides that trigger [[PrkC]] activity to the expression of the genes of the [[WalR regulon]] that are involved in [[cell wall synthesis|cell wall metabolism]].
 
* '''Relevant ''Subti''Wiki pages:''' [[Jonathan Dworkin]], [[Protein kinases and phosphatases|protein kinases]], [[YabT]], [[TufA|EF-Tu]], [[PrkC]], [[WalR]], [[cell wall synthesis|cell wall metabolism]]
 
* '''Relevant ''Subti''Wiki pages:''' [[Jonathan Dworkin]], [[Protein kinases and phosphatases|protein kinases]], [[YabT]], [[TufA|EF-Tu]], [[PrkC]], [[WalR]], [[cell wall synthesis|cell wall metabolism]]

Revision as of 08:30, 6 August 2015

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Paper of the month: July 2015

Elizabeth A Libby, Lindsie A Goss, Jonathan Dworkin
The Eukaryotic-Like Ser/Thr Kinase PrkC Regulates the Essential WalRK Two-Component System in Bacillus subtilis.
PLoS Genet: 2015, 11(6);e1005275
[PubMed:26102633] [WorldCat.org] [DOI] (I e)

Sandro F F Pereira, Ruben L Gonzalez, Jonathan Dworkin
Protein synthesis during cellular quiescence is inhibited by phosphorylation of a translational elongation factor.
Proc Natl Acad Sci U S A: 2015, 112(25);E3274-81
[PubMed:26056311] [WorldCat.org] [DOI] (I p)


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News in SubtiWiki:

  • August 2014: The first mobile application for SubtiWiki is now available for users of Android phones: Go for SubtiWiki in Google Play! IPhone users, please be patient! You will be served next! Click here to install!
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  • March 2013: updated Excel files that map all genes to categories and regulons as well as the general updated identity mapping of all genes were uploaded.
  • October 2012: the essential genes have been categorized
  • September 2012: 300 mutant strains available at the BGSC were added to the corresponding gene pages
  • August 2012: SubtiExpress, the new compagnon of SubtiWiki devoted to gene expression is online! Try it out!
  • April 2012: Links for expression data under 104 different conditions (based on Nicolas et al., 2012) have been added to the expression section of the individual gene pages
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Genes (examples) Additional pages
spo0A SubtiPathways
glnA SubtInteract
rny SubtiExpress
psdA Regulons
pckA methods
dnaA metabolism
rnpA categories
sigB genome-wide analyses
ptkA list of plasmids
mreC labs working on Bacillus
ccpA the people behind SubtiWiki
sr1 template


Publications describing SubtiWiki and SubtiPathways: