MaeA

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  • Description: malic enzyme

Gene name maeA
Synonyms ywkA
Essential no
Product NAD-dependent malate dehydrogenase
Function malate utilization
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 63 kDa, 4.883
Gene length, protein length 1746 bp, 582 aa
Immediate neighbours ywkB, tdk
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwkA context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU37050

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-malate + NAD+ = pyruvate + CO2 + NADH (according to Swiss-Prot) malate --> pyruvate
  • Protein family: malic enzymes family (according to Swiss-Prot)
  • Paralogous protein(s): MalS

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of malate (MalR) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1141 (aphA3) available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References

Guillaume Lerondel, Thierry Doan, Nicola Zamboni, Uwe Sauer, Stéphane Aymerich
YtsJ has the major physiological role of the four paralogous malic enzyme isoforms in Bacillus subtilis.
J. Bacteriol.: 2006, 188(13);4727-36
[PubMed:16788182] [WorldCat.org] [DOI] (P p)

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading, Engl.): 2003, 149(Pt 9);2331-43
[PubMed:12949160] [WorldCat.org] [DOI] (P p)