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  • Description: gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)

Gene name lytF
Synonyms cwlE, yhdD
Essential no
Product gamma-D-glutamate-meso-diaminopimelate muropeptidase (major autolysin)
Function cell separation
MW, pI 51 kDa, 10.41
Gene length, protein length 1464 bp, 488 aa
Immediate neighbours yhdC, nsrR
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
LytF context.gif
This image was kindly provided by SubtiList

The gene

Basic information

  • Locus tag: BSU09370

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: nlpC/p60 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions: IseA-LytF (catalytic domain)
  • Localization: secreted (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks


Rui Chen, Sarah B Guttenplan, Kris M Blair, Daniel B Kearns
Role of the sigmaD-dependent autolysins in Bacillus subtilis population heterogeneity.
J Bacteriol: 2009, 191(18);5775-84
[PubMed:19542270] [] [DOI] (I p)

Hiroki Yamamoto, Yukiko Miyake, Miharu Hisaoka, Shin-Ichirou Kurosawa, Junichi Sekiguchi
The major and minor wall teichoic acids prevent the sidewall localization of vegetative DL-endopeptidase LytF in Bacillus subtilis.
Mol Microbiol: 2008, 70(2);297-310
[PubMed:18761696] [] [DOI] (I p)

Hiroki Yamamoto, Shin-ichirou Kurosawa, Junichi Sekiguchi
Localization of the vegetative cell wall hydrolases LytC, LytE, and LytF on the Bacillus subtilis cell surface and stability of these enzymes to cell wall-bound or extracellular proteases.
J Bacteriol: 2003, 185(22);6666-77
[PubMed:14594841] [] [DOI] (P p)

R Ohnishi, S Ishikawa, J Sekiguchi
Peptidoglycan hydrolase LytF plays a role in cell separation with CwlF during vegetative growth of Bacillus subtilis.
J Bacteriol: 1999, 181(10);3178-84
[PubMed:10322020] [] [DOI] (P p)

Philippe Margot, Marco Pagni, Dimitri Karamata
Bacillus subtilis 168 gene lytF encodes a gamma-D-glutamate-meso-diaminopimelate muropeptidase expressed by the alternative vegetative sigma factor, sigmaD.
Microbiology (Reading): 1999, 145 ( Pt 1);57-65
[PubMed:10206711] [] [DOI] (P p)