Difference between revisions of "List of plasmids"

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* [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
 
* [[pGP1331]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
 
* [[pGP1087]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
 
* [[pGP1087]]: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
 +
* [[pGP1751]]: ''pckA'' ''SalI'' + ''SphI'' cloned into [[pGP1331]]
  
 
==Expression/ purification in ''B. subtilis''==
 
==Expression/ purification in ''B. subtilis''==
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*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein
 
*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein
 
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
 
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
 +
* [[pGP1753]]: ''pckA'' ''XbaI'' + ''PstI'' cloned into [[pGP380]]
  
 
==Expression/ purification in ''E. coli''==
 
==Expression/ purification in ''E. coli''==

Revision as of 14:57, 5 May 2010

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

  • pAC5: translational lacZ-fusions (chloramphenicol resistence)
  • pAC6: transcriptional lacZ-fusions (cat resistence)
  • pAC7: translational lacZ-fusions (kanamycin resistence)
  • pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed

Labelling of a protein with a triple FLAG tag

  • pGP1331: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter
  • pGP1087: integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping expression under the control of the natural promoter, allowing the expression of downstream genes under the control of the IPTG-inducible Pspac promoter
  • pGP1751: pckA SalI + SphI cloned into pGP1331

Expression/ purification in B. subtilis

  • pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
  • pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein
  • pGP1753: pckA XbaI + PstI cloned into pGP380

Expression/ purification in E. coli

  • pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
  • pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
  • pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein
  • pGP1755: expression of a mutated malatedehydrogenase from B. subtilis in E. coli; mdh (Ser-149-Ala) Bam+Pst in pWH844