Difference between revisions of "List of plasmids"

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Here you can find a list of ''Bacillus subtilis'' plasmids:
 
Here you can find a list of ''Bacillus subtilis'' plasmids:
  
 +
==Fusion to reporter genes==
 
*[[pAC5]]: translational ''lacZ''-fusions (chloramphenicol resistence)
 
*[[pAC5]]: translational ''lacZ''-fusions (chloramphenicol resistence)
 
*[[pAC6]]: transcriptional ''lacZ''-fusions (cat resistence)
 
*[[pAC6]]: transcriptional ''lacZ''-fusions (cat resistence)
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* pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}}
 
* pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression {{PubMed|20150235}}
  
 +
==Expression/ purification in ''B. subtilis''==
 
*[[pBQ200]]: overexpression of proteins in ''B. subtilis'' under control of a strong ''degQ36'' promoter
 
*[[pBQ200]]: overexpression of proteins in ''B. subtilis'' under control of a strong ''degQ36'' promoter
 
*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein
 
*[[pGP380]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the N-terminus of the protein
 
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
 
*[[pGP382]]: expression of proteins in ''B. subtilis'' allows fusion to a Strep-tag at the C-terminus of the protein
  
 +
==Expression/ purification in ''E. coli''==
 
*[[pWH844]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag
 
*[[pWH844]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag
 
*[[pGP570]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag that can be cleaved off
 
*[[pGP570]]: expression of proteins in ''E. coli'', allows fusion to a N-terminal His-tag that can be cleaved off
 
 
*[[pGP172]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the N-terminus of the protein
 
*[[pGP172]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the N-terminus of the protein
 
*[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein
 
*[[pGP574]]: expression of proteins in ''E. coli'' allows fusion to a Strep-tag at the C-terminus of the protein

Revision as of 11:46, 11 March 2010

Here you can find a list of Bacillus subtilis plasmids:

Fusion to reporter genes

  • pAC5: translational lacZ-fusions (chloramphenicol resistence)
  • pAC6: transcriptional lacZ-fusions (cat resistence)
  • pAC7: translational lacZ-fusions (kanamycin resistence)
  • pBaSysBioII: gfp transcriptional fusions for high-throughput analysis of gene expression PubMed

Expression/ purification in B. subtilis

  • pBQ200: overexpression of proteins in B. subtilis under control of a strong degQ36 promoter
  • pGP380: expression of proteins in B. subtilis allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP382: expression of proteins in B. subtilis allows fusion to a Strep-tag at the C-terminus of the protein

Expression/ purification in E. coli

  • pWH844: expression of proteins in E. coli, allows fusion to a N-terminal His-tag
  • pGP570: expression of proteins in E. coli, allows fusion to a N-terminal His-tag that can be cleaved off
  • pGP172: expression of proteins in E. coli allows fusion to a Strep-tag at the N-terminus of the protein
  • pGP574: expression of proteins in E. coli allows fusion to a Strep-tag at the C-terminus of the protein